von Willebrand Factor Collagen Binding
General Information
- Lab Name
- VW Collagen Binding
- Lab Code
- VWFCBA
- Epic Ordering
- Von Willebrand Factor Collagen Binding
- Description
The von Willebrand Factor (VWF) Collagen Binding test is used to measure the adhesive properties of the VWF to collagen. Patient's plasma is incubated in the wells of microtiter plates that have been coated with human collagen type III. After washing off non-adherent proteins, bound von Willebrand factor is quantified using anti-VWF antibodies with chromogenic detection.
The von Willebrand Factor Collagen Binding test is proven to be more precise and more reliable in the diagnosis of type 1 vWD when compared to the ristocetin cofactor assay. Although the presence of decreased vWF:CBA is suggestive of von Willebrand disease, the assay must not be used alone but rather in conjunction with a group of tests (vWF antigen and activity, Factor VIII activity and multimer analysis) as well as other clinical findings to make the final diagnosis.
- Synonyms
- Components
-
Code Name VWFCB VWF Collagen Binding VWCBM VWF Collagen Binding Method
Interpretation
- Method
Enzyme Linked Immunosorbent Assay (ELISA).
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
2, 3 or 5 mL BLUE TOP (CITRATE) tube
- Handling Instructions
The Laboratory MUST process specimen within 4 hours of collection.
- Quantity
-
requested: Entire sample
minimum: 0.5 mL plasma
Processing
- Processing
UW-MT: Take specimen to UW-MT Coag lab for processing. Coag tech will freeze plasma sample for transport to HMC Coag.
HMC: Take specimen to HMC Coag lab for processing.
Outside Laboratories: Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 0.5 mL) @ -20°C to -80°C. Send frozen on dry ice.
Performance
- LIS Dept Code
- Coagulation (COAG)
- Performing Location(s)
-
HMC Coagulation
206-520-4600325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
- Frequency
- Run on Tuesday and Friday. Reported by end of day.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 83520
- LOINC
- 50377-1
- Interfaced Order Code
- UOW3499