von Willebrand Factor Collagen Binding

General Information

Lab Name

VW Collagen Binding

Lab Code

VWFCBA

Epic Name

Von Willebrand Factor Collagen Binding

Description

The von Willebrand Factor (VWF) Collagen Binding test is used to measure the adhesive properties of the VWF to collagen. Patient's plasma is incubated in the wells of microtiter plates that have been coated with human collagen type III. After washing off non-adherent proteins, bound von Willebrand factor is quantified using anti-VWF antibodies with chromogenic detection.

The von Willebrand Factor Collagen Binding test is proven to be more precise and more reliable in the diagnosis of type 1 vWD when compared to the ristocetin cofactor assay. Although the presence of decreased vWF:CBA is suggestive of von Willebrand disease, the assay must not be used alone but rather in conjunction with a group of tests (vWF antigen and activity, Factor VIII activity and multimer analysis) as well as other clinical findings to make the final diagnosis.

Components

Code	Name
VWFCB	VWF Collagen Binding
VWCBM	VWF Collagen Binding Method