HSV Acyclovir Drug Resistance by Sanger Sequencing

General Information

Lab Name

HSV Acyclovir Drug Resistance

Lab Code

HSVDR

Epic Ordering

HSV Acyclovir Drug Resistance

Description

The test is designed to detect and characterize Acyclovir drug resistance associated with specific mutations found in the Thymidine Kinase (UL23) gene of both HSV Type 1 and HSV Type 2. These mutations can lead to a reduced or absent response to Acyclovir, a commonly used antiviral treatment.

This test was developed, and its performance characteristics were determined, by the University of Washington Department of Laboratory Medicine and Pathology. It is important to note that while this test has been validated for clinical use, it has not been cleared or approved by the US Food and Drug Administration (FDA). However, under the Clinical Laboratory Improvement Amendments (CLIA), the University of Washington laboratory ensures the test meets high standards for accuracy and reliability in a clinical setting.

Synonyms

Acyclovir, Drug Resistance, HSV, Sanger

Components

Code	Name
HSVSPC	HSV Acyclovir Sample Type
HSVDRM	HSV Mutations Associated with Acyclovir Resistance
HSVNDR	Other HSV Mutations Causing Amino Acid Changes
HSVACY	HSV Acyclovir Resistance
HSVDRI	HSV Acyclovir Resistance Interpretation

Interpretation

Method: Sanger Sequencing
Reference Range: See individual components

Ordering & Collection

Specimen Type

Swabs, Blood, Spinal Fluid, BAL, Eye Fluid

Collection

Collect ONE of the following:

Swabs in 1.5 mL VTM
3 mL blood in LAVENDER, RED, SST, PPT tube
1 mL Spinal Fluid
1 mL BAL

Unacceptable: GREEN TOP, Extracted nucleic acid from an outside laboratory.

Handling Instructions

Quantity

requested: 1.5 mL plasma/serum/CSF/BAL or swabs in 1.5 mL VTM
minimum: 1 mL plasma/serum/CSF/BAL or swabs in 1 mL VTM

Processing

Processing: Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

CSF or Clear non-cellular fluid: If CSF has cellular material, centrifuge & aliquot clear CSF. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code

Virology, Eastlake (EVIR)

Performing Location(s)

Other	Virology, Molecular 206-685-6656 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102

Frequency

Once a week

Available STAT?

Billing & Coding

CPT codes: 87900
LOINC: 29578-2
Interfaced Order Code: UOW3692