HIV Genotypic Resistance Assay
General Information
- Lab Name
- HIV Genotypic Resistance Assay
- Lab Code
- HIVGRA
- Epic Ordering
- HIV Genotypic Resistance Assay
- Description
HIV genotypic testing for resistance assesses the genotype of a patient’s HIV status and indicates which drugs the patient’s HIV is likely to be resistant to. This provides a helpful guide for the clinician to provide appropriate anti-retroviral therapy for the patient. Viral RNA is amplified into DNA, cleaned, and then sequenced using fluorescein-labelled dyes. Raw sequenced data is further analyzed to construct the genotype of the patient’s HIV1. The Pol gene encodes enzymes including the Protease, Reverse transcriptase and Integrase. This genotype is then compared to a wild-type HIV sequence, and the mutations noted. All mutations are listed in a report. The report also includes an interpretation of the mutation pattern in order to give a resistance profile of the patient’s HIV.
Specimens with RNA loads<40 copies/ml will be rejected prior to performing RNA extraction. Approximately 1000 copies/mL are required to yield a sequenceable PCR amplicon.
This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.
- Synonyms
- HIV Antiviral Resistance Testing, HIVGRR
- Components
-
Code Name HVGPAA HIVGR, Protease Amino Acids HVGPRM HIVGR, Prot: Resist Mutations HVGPOM HIVGR, Prot: Other Mutations HVGTAA HIVGR, RT: Amino Acids HVGTRM HIVGR, RT: Resist Mutations HVGTOM HIVGR, RT: Other Mutations HIVGRI HIV Interp of Genotype HVGPI Protease Inhibitors HGATV Atazanavir (Reyataz) ATV HGDRV Darunavir (Prezista) DRV HGLPV Lopinavir_ritonavir (Kaletra) LPV HGNRI Nucleoside/Nucleotide RT Inhibitors HGABC Abacavir (Ziagen) ABC HGAZT Zidovudine (Retrovir) ZDV/AZT HGFTC Emtricitabine (Emtriva) FTC HG3TC Lamivudine (Epivir) 3TC HGTDF Tenofovir (Viread) TDF/TAF HGNNRI Non Nucleoside RT Inhibitors HGDOR Doravirine (Pifeltro) DOR HGEFV Efavirenz (Sustiva) EFV HGETR Etravirine (Intelence) ETR HGNVP Nevirapine (Virammune) NVP HGRPV Rilpivirine (Edurant) RPV HGCOM HIVGR, Comment
Interpretation
- Method
Sequencing/Real-time Polymerase Chain Reaction.
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Blood: 6 mL blood in EDTA (LAVENDER TOP), SST, RED, or PEARL TOP (PPT).
Note: HIVGRA and HVINTA all test can be done on same 2 mL aliquot.
Unacceptable: GREEN TOP, Extracted nucleic acid from an outside laboratory.
Minimum sample requirement: 2 mL
- Handling Instructions
- Quantity
-
requested: 2 mL plasma/serum
minimum: 2 mL plasma/serum
Processing
- Processing
Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Outside Laboratories: Centrifuge sample and remove plasma/serum. Send Frozen plasma/serum at -10°C or colder and ship on dry ice.
Performance
- LIS Dept Code
- Clinical Virology (CVIR)
- Performing Location(s)
-
Other Virology
206-685-8037Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102 - Frequency
- Wednesday set up. Results 14-21 days later.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 87901
- LOINC
- 49660-4
- Interfaced Order Code
- UOW1051