HBV Drug Resistance Sequencing

General Information

Lab Name

Lab Code

HBVDR

Epic Ordering

HBV Drug Resistance Sequencing

Description

Sanger sequencing of the HBV polymerase gene is used to determine the HBV genotype and to identify drug resistant mutations. An 850bp region of the HBV DNA polymerase gene, amino acids 25 to 344, is sequenced and compared to an HBV genotyping database provided by Genofar, a German, non-profit, scientific society. This test may be added-on to a positive HBV DNA quantitative PCR assay (HBVQNT), call the Virology Laboratory at 206-685-6656 to confirm sample availability. If both HBV Drug Resistance and Genotype testing are requested, please order HBVGTD.

Synonyms

HBV Drug Resistance, HBV POL Gene Sequencing, Hepatitis B Drug Resistance

Components

Code	Name
HBVSD	HBV Specimen Description
HBVDRM	HBV Drug Resistance Mutation
HBVLAM	HBV Lamivudine Resistance
HBVADE	HBV Adefovir Resistance
HBVENT	HBV Entecavir Resistance
HBVTEN	HBV Tenofovir Resistance
HBVTEL	HBV Telbivudine Resistance
HBVINT	HBV Generic Interpretation

Interpretation

Method: Real-Time Polymerase Chain Reaction and Sanger sequencing
Reference Range: See individual components

Ordering & Collection

Specimen Type: Blood
Collection: Blood: 3 mL blood in LAVENDAR, RED, GOLD SST, or PPT (pearl top).

Unacceptable samples: Plasma collected in sodium or lithium heparin.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Minimum sample requirement: 0.7 mL
Handling Instructions
Quantity: requested: 1 mL
minimum: 0.7 mL

Processing

Processing: Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code

Virology, Eastlake (EVIR)

Performing Location(s)

Other	Virology, Molecular 206-685-6656 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102

Frequency

Tuesday set-up, results by Friday

Available STAT?

Billing & Coding

CPT codes
LOINC: 49883-2
Interfaced Order Code: UOW1526