AFB Culture, Blood

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General Information

Lab Name
AFB Culture, Blood
Lab Code
AFBBC
Epic Name
AFB Culture, Blood
Description

See AFB Culture, Bone Marrow [AFBBNM] for AFB culture on bone marrow.

Synonyms
Acid Fast, AFB, MAC, MTB, Mycobacteria, TB, Tuberculosis
Components
Code Name
SDES Specimen Description
SREQ Special Requests
CULT Culture
RPT Report Status

Interpretation

Method

BD BACTEC™ Myco/F Lytic culture vials

BD BACTEC™ FX40 System for Mycobacteria Blood Cultures

Reference Range
See individual components

Ordering & Collection

Specimen Type
Blood
Collection

Disinfect draw site and TUBE TOP. Allow disinfectant to dry. Collect blood in a yellow-top BD Vacutainer Tube containing SPS. Do NOT overfill or underfill. Inoculate the maximum volume for optimal sensitivity of culture.

Unacceptable:

  • Any anticoagulant other than SPS
  • Specimens older than 15 hrs will be rejected

HMC: Contact HMC Microbiology at 206-744-5858 for SPS tube. Refer to "Nursing Services Policy and Procedures for Nursing Care: Obtaining Blood Culture" procedure.

UW-MT: SPS tubes are available from Material Management. Refer to "Nursing Services Policy and Procedures for Nursing Care: Obtaining Blood Culture" procedure. Specimen will be sent to HMC for testing.

UW-NW: Contact UW-NW specimen processing at 206-668-2026 for SPS tube. Specimen will be sent to HMC for testing.

Handling Instructions

Transport at room temperature to Microbiology laboratory within 30 minutes after collection. Specimens older than 15 hours will be rejected. Specimen must be received in HMC Microbiology BEFORE 10:30 pm for same day processing.

Quantity
Requested: 8.3 mL blood
Minimum: 1 mL Blood

Processing

Receiving Instructions

Specimens received from outside locations and clinics must be sent to the HMC Microbiology lab immediately for processing. At login, enter the phlebotomy site.

Misc Sendout

Performance

Lab Department
Microbiology(MC)
Frequency
Daily: 7:00 - 22:30 [Midnight Shift: leave SPS tube at room temperature and leave a message to alert Dayshift Microbiology.]
Available STAT?
No
Performing Location(s)
HMC Microbiology
206-744-5858

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
Other Locations/Notes

HMC Microbiology: Inoculate blood into a BACTEC Myco/F Lytic culture vial. See lab procedure “BD BACTED FX40 System for Mycobacteria Blood Cultures” for further directions.

Billing & Coding

CPT Codes
87015, 87116
LOINC
64412-0
Interfaced Order Code
UOW1037