Adenovirus Shell Vial

General Information

Lab Name

Lab Code

ADSV

Epic Name

Adenovirus Shell Vial

Description

NOTE: This test is temporarily unavailable as of 03/25/2020.

The detection of viable Adenovirus (ADV) prior to visible cytopathic effect (CPE) in conventional tube tissue culture. Specimens are inoculated into two 48-well plates containing a monolayer of a receptive cell line and centrifuged at low speed. At day 1 and 3 post inoculation, one plate is stained with a monoclonal antibody to adenovirus, and then reacted with fluorescent-conjugated goat anti-mouse immunoglobulin G. The cells are examined for characteristic nuclear staining which indicates the presence of adenovirus growth. Results are reported as ADENOVIRUS DETECTED or Not detected.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

Only use this test code in conjunction with a Viral Culture Screen Viral Culture Screen [VCS].

Components

Code	Name
ADSVSD	ADSV specimen description
ADSVD1	ADSV Result DAY 1
ADSVD3	ADSV Result DAY 3

Interpretation

Method: Tissue culture.
Reference Range: See individual components

Ordering & Collection

Specimen Type: Body fluids (CSF, BAL, Washes, Urine, Sputum), Swabs, Tissue, and Stool
Collection: Body fluids (CSF, BAL, Washes, Urine, Sputum): 1 mL of sample sterile container

NP/Conjunctiva swabs: use FLEXIBLE, MINITIP FLOCKED SWAB in 3 ml universal transport media (UTM)
UWMC - PMM 304528, HMC - 51264, Community - Copan 305C or DHI 403C

Other Swabbed sites: use REGULAR FLOCKED SWAB in 3 ml universal transport media UTM

Tissue: in sterile saline/universal transport media (UTM)

Stool: pea size sample collected in a sterile container
Quantity: Requested: See Collection
Minimum: 1 mL

Processing

Receiving Instructions: Body fluids (CSF, BAL, Washes, Urine, Sputum): Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

NP/Conjunctiva swabs: Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

Other Swabbed sites: Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

Tissue: Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

Stool: Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -70°C or colder. Do not freeze/thaw.

Outside Laboratories:

Ship samples on Dry Ice, do not freeze/thaw. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack), shipment time must not exceed 48 hours from collection. Samples stored at 2-8°C greater than 48 hours old may be rejected.

Unacceptable samples: Blood (serum/plasma), dry swabs.

Note: Freezing specimens at -20°C is NOT acceptable for viral culture.

Misc Sendout

Performance

Lab Department

Virology - OLD(VIRO)

Frequency

Monday-Friday: Specimen in lab by 5PM. Saturday, Sunday and Holidays: Specimen in Lab by 2PM

Available STAT?

Performing Location(s)

Other	Virology 206-685-8037 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102

Billing & Coding

LOINC: 50024-9
Interfaced Order Code: UOW1174