ABL1 Kinase Mutation Analysis

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General Information

Lab Name
Lab Code
992
Description

Mutations in the ABL1 kinase domain may cause, or contribute to, resistance to tyrosine kinase inhibitors (TKIs) in chronic myeloid leukemia patients or Ph+positive B lymphoblastic leukemia.

If indicated, sequencing of ABL1 gene is available to order as a heme single gene by NGS (HCAPSG – ABL1) or is also available in the myeloid-focused panel (HCAPMY).

Use mnemonic HCAPSG to order this test.

Forms & Requisitions

Hematopathology Requisition

Synonyms
ABL1 Kinase Mutation, HCAPSG, Heme Single Gene by NGS
Components

Interpretation

Method

Next-Generation Sequencing

Reference Range

Ordering & Collection

Specimen Type
Blood, Bone Marrow, Tissue, Fluid, Slides, Sorted Cells
Collection

Preferred:

  • Blood: 6mL in Lavender Top (EDTA)

  • Bone Marrow: 1-2mL in Lavender Top (EDTA)

  • Tissue, Fluids or Sorted cells:

    • Sterile container in RPMI

    • Formalin-fixed paraffin embedded tissue (FFPE/PET) block

  • Slides: 10 unstained

Accepted:

  • Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.

  • Extracted DNA from CLIA certified laboratory.

  • Formalin-fixed paraffin embedded tissue (FFPE/PET)

  • Tissue from curls cut from FFPE/PET blocks or stained cover-slipped slides

Unacceptable:

  • Specimens received beyond stability limit.
  • Specimens with low volume or inadequate WBC
  • Decalcified or unacceptable fixatives
Forms & Requisitions

Hematopathology Requisition

Handling Instructions

Samples should be transported at room temperature and arrive in lab within 48 hours.

If sample is expected to arrive in the HP lab beyond 48 hours, store and transport at 2 – 6˚C. The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.

Frozen samples should be shipped on dry ice.

Quantity
Requested: Blood: 6mL EDTA/BMA: 1-2mL EDTA/Tissue Blocks: Adequate size to cut 10-20 curls/Slides: 10 unstained
Minimum: Blood: 1mL EDTA/ BMA: 0.5mL EDTA/Tissue Blocks: 5 curls/Slides: 5 slides

Processing

Receiving Instructions

Send specimens to Hematopathology – Molecular department for processing.

The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.

Blood and BMA must arrive in lab by 7am and tissue by 12pm Friday to be included on Monday run.

Misc Sendout

Performance

Lab Department
Frequency
Monday: Results within 12 - 14 days from start of testing.
Available STAT?
No
Performing Location(s)
Other Hematopathology, Molecular
206-606-7060

UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109

Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays

Billing & Coding

Billing Comments

CPT: 81479