SARS-CoV-2 Whole Genome Sequencing Assay – Strain typing and resistance testing

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General Information

Lab Name
SARS CoV 2 NGS Variant Analysis
Lab Code
Epic Ordering
SARS CoV 2 NGS Variant Analysis

This assay recovers the whole genome sequence of SARS-CoV-2 and reports the SARS-CoV-2 strain, including variants of concern, as well as specific coding mutations found in the SARS-CoV-2 spike protein relative to the Wuhan-Hu1 isolate SARS-CoV-2 reference sequence. The limit of detection of the assay is Ct 33.

COVID, SARS CoV 2 Spike Mutations, SARS CoV 2 Variant Mutations, SARS CoV 2 Variant Sequencing, SARS CoV 2 Variants


Reference Range
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Ordering & Collection

Specimen Type

Instructions for Nasopharyngeal Swab: Gently insert mini-tipped flocked nasopharyngeal swab (swab on flexible plastic shaft) through the nostril and into the nasopharynx, reaching the posterior nasopharynx. Leave swab in place for 2-3 seconds then rotate completely around for 10-15 seconds. Remove swab and repeat the same process in the other nostril with the same swab.
After the second swab is completed, immediately place into the sterile vial containing media (UTM is preferred). Make sure that the swab is fully immersed in media, and that the shaft is short enough to completely tighten the cap. Obtaining columnar epithelial cells will enhance reliability of viral detection.

Handling Instructions

Send to the laboratory as soon as possible. Sample may be stored at 2-8°C for up to 72 hours of collection. Ship immediately to lab at 2-8°C (ice pack). If collection to receipt in the lab will exceed 72 hours freeze at -10°C or colder and ship on dry ice. Do not freeze/thaw.

General instructions for specimen collection and shipping: Specimen Collection

requested: 0.5mL
minimum: 0.5mL



Sample must be positive for SARS-CoV-2 by PCR to proceed with testing. Transport and store tube at 2 to 25°C for up to 48 hours. Send to UW Virology Lab via courier.

Transport Temperature


LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Eastlake

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102

Available STAT?

Billing & Coding

CPT codes