Quantitative Respiratory virus follow-up by PCR

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General Information

Lab Name
Resp Virus PCR Quant Follow-up
Lab Code
RVFQNT
Epic Ordering
Resp Virus PCR Quant Follow-Up
Description

The UW LDT Respiratory virus panel follow up by quantitative real-time (RT-PCR) assay is intended to monitor positive patients who are being treated for Influenza types A and B, Metapneumovirus (MPV), Respiratory Syncytial Virus (RSV) and Parainfluenza type 3 (PIV3) with an antiviral drug. Specimens will be accepted only from patients who tested positive within the previous month.

The following viruses are targeted:

  • Influenza type A (matrix)
  • Influenza type B (matrix)
  • Metapneumovirus, MPV (fusion)
  • Respiratory Syncytial Virus, RSV (matrix)
  • Parainfluenza type 3, PIV3 (matrix)

These patients may have tested positive by REVSQT, FLUPCR, RVFQNT, FLURSV, NCVFLU viral culture, or a rapid test. The minimum virus level that gives a positive result in 95% of replicates is 1,000 copies/mL (3 log copies/mL). Result are reported as “not detected”, “positive”, or quantity in copies/mL if result is >1000 copies/mL.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

Synonyms
quantitative influenza A PCR, quantitative influenza B PCR, quantitative MPV PCR, quantitative Parainfluenza type 3 PCR, quantitative RSV PCR
Components

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range
See individual components

Ordering & Collection

Specimen Type
BAL, Washing, Swab, or Aspirate. Note: Need approval from Laboratory Medicine Resident for Serum, Plasma, CSF, Body Fluid, or Tissue. Specimens will be accepted only from patients who were tested positive within the previous month for: •Influenza type A •Influenza type B •Metapneumovirus (MPV) •Respiratory Syncytial Virus (RSV) •Parainfluenza type 3 (PIV3)
Collection

BAL, Washing, Swab, or Aspirate: 1 mL in sterile tube.

Serum, Plasma, CSF, or Body Fluid: 1 mL in sterile tube.

Tissue: Pea size amount in sterile tube or universal transport media (UTM).

Minimum sample requirement: 0.5 mL

Handling Instructions

Refrigerate within 4 hours of collection. Send refrigerated specimen at 2 to 8°C to lab on ice packs within 48 hours of collection. Specimens frozen at -20°C are acceptable on dry ice.

Quantity
requested: See Collection
minimum: 0.5 mL

Processing

Processing

Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 48 hours of collection. If time of collection exceeds 48 hours, freeze sample at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Molecular
206-685-6656

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102
Frequency
Monday – Friday. Results same day.
Available STAT?
No

Billing & Coding

CPT codes
LOINC
50548-7
Interfaced Order Code
UOW1830