HBV Genotype by Sequencing
General Information
- Lab Name
- HBV Genotype by Sequencing
- Lab Code
- HBVGT
- Epic Ordering
- HBV Genotype Sequencing
- Description
HBV Drug Resistance is determined by Sanger sequencing. An 850bp region of the HBV DNA polymerase gene, amino acids 25 to 344, is sequenced and compared to an HBV drug resistant database provided by Genofar, a German, non-profit, scientific society. PCR sensitivity is 75 IU/mL. Minor subpopulations of mutant viruses below 20-25% of the total HBV population may not be detected. Drug resistance analysis includes Lamivudine, Adefovir, Entecavir, Tenofovir and Telbivudine. This test may be added-on to a positive HBV DNA quantitative PCR assay (HBVQNT), call the Virology Laboratory, (206) 685-6656, to confirm sample availability. If both HBV Drug Resistance and Genotype testing are requested, please order HBVGTD.
- Synonyms
- HBV Genotype, Hepatitis B Genotype
- Components
-
Code Name HBVSD HBV Specimen Description HBVGER HBV Genotype Result HBVGEI HBV Genotype Interpretation
Interpretation
- Method
Real-Time Polymerase Chain Reaction/Sanger sequencing
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Blood: 3 mL blood in LAVENDAR, RED, GOLD SST, or PPT (pearl top).Plasma (ACD, EDTA, CPD, and plasma preparation tubes) or serum (including serum collected in serum separator tubes).
Unacceptable specimen: Plasma collected in sodium or lithium heparin (green top).
Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.
- Handling Instructions
- Quantity
-
requested: 1 mL
minimum: 0.7 mL
Processing
- Processing
Blood: Separate plasma from whole blood within 24 hours of collection. Freeze plasma at -10°C or colder and ship on dry ice. Plasma may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Do not freeze/thaw.
Outside Laboratory Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Performance
- LIS Dept Code
- Virology, Eastlake (EVIR)
- Performing Location(s)
-
Other Virology, Molecular
206-685-6656Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102 - Frequency
- Tuesday set-up, results by Friday
- Available STAT?
- No
Billing & Coding
- CPT codes
- LOINC
- 32366-7
- Interfaced Order Code
- UOW1533