JC Virus by PCR Quantitative, Urine
- Lab Name
- JCV PCR Quant, URN
- Lab Code
- Epic Ordering
- JCV Quantitative PCR, Urine
The detection and quantification of JC Virus (JCV) by real-time PCR amplification. The primers used in this PCR reaction amplify the Large T antigen gene. The JC Virus probes are labeled with reporter dye FAM and quencher TAMRA. Quantification of JC Virus DNA (copies/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the JC Virus amplicons. The minimum virus level that gives a positive result in 95% of replicates is 500 copies/mL (2.70 log copies/mL).
This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.
For Blood, CSF, or Clear non-cellular fluid (ex: BAL, or Washing), see JC Virus by PCR Quantitative [JCVQN].
For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (ex: BAL or Washing), see JC Virus Qualitative PCR, Tissue [JCVQLT].
- JC Virus, PML Virus, Progressive Multifocal Leukoencephalopathy virus
Real-time Polymerase Chain Reaction
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
Urine: 10 mL Random or Clean catch urine in sterile container.
Note: Separate specimens MUST be submitted when both Viral Culture or Serology and PCR testing are ordered.
Unacceptable Samples: Urine with Preservative.
- Handling Instructions
requested: 10 mL Urine
minimum: 5 mL Urine
Urine: Send urine at 2-8°C (ice pack) within 48 hours of collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -10°C or colder. Do not freeze/thaw.
Outside Laboratories: Ship samples on dry ice, do not freeze/thaw. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack), shipment time must not exceed 48 hours from collection. Samples stored at 2-8°C greater than 48 hours old may be rejected.
- Transport Temperature
- LIS Dept Code
- Virology, Eastlake (EVIR)
- Performing Location(s)
Other Virology, Molecular
Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102
- Tuesday. Results same day.
- Available STAT?
Billing & Coding
- CPT codes