EBV Quant by PCR, Blood Spots

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General Information

Lab Name
EBV Quant by PCR, Blood Spots
Lab Code
EBVBSQ
Epic Ordering
EBV Quantitative PCR, Dried Blood Spots
Description

The detection and quantitation of Epstein Barr Virus (EBV) by real-time PCR amplification. The primers in this EBV PCR reaction amplify a fragment of the EBV IR1 gene. This assay is used for dried blood spots and amplifies a fragment of the EBV Internal repeats within the EBNA region. Quantitation of EBV DNA (IU/mL) is achieved by amplifying a standard curve consisting of serial dilutions of a known amount of DNA containing the EBV amplicons. The minimum virus level that gives a positive result in 95% of replicates is 100 IU/mL in blood (2.0 log IU/ml).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF, Swab, Eye Fluid or Clear non-cellular fluid (e.g. BAL or Washing), see EBV Quantitative by PCR [EBVQ].

For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (e.g. BAL or Washing), see EBV by PCR Qualitative, Tissue /Cells [EBVQLT].

Synonyms
Components

Interpretation

Method

Real-time Polymerase Chain Reaction

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Dried Blood Spot
Collection

Dried Blood Spot: Testing only performed on the original Newborn Screening Card from patient's time of birth. See Specimen Handling to request card from WA DOH.

Fill one circle of a Newborn Screening Card completely with blood (confirm blood has soaked through the card). Label or tag each specimen in English with at least two of the following: patient name, date of birth and/or unique I.D. number corresponding to the request form.

Minimum sample requirement: 1 full circle

Handling Instructions

In-house:

The original Newborn Screening Card must be obtained from the WA DOH using the In-house Consent Form.

In-house providers must place future orders for testing in EPIC

Outside:

Obtain the original Newborn Screening Card from the DOH using the CSS/Ref Consent Form. Once specimen is received, forward to UWMC SPS with completed Virology Lab Request Form.

Quantity
requested: 1 full circle
minimum: 1 full circle

Processing

Processing

Send blood spot to Molecular Virology lab at room temperature.

Transport Temperature

Performance

LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Molecular
206-685-6656

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102

Frequency
Friday. Results same day.
Available STAT?
No

Billing & Coding

CPT codes
87799
LOINC
53774-6