CMV Quant by PCR, Blood Spots

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General Information

Lab Name
CMV Quant by PCR, Blood Spots
Lab Code
Epic Ordering
CMV Quantitative PCR, Dried Blood Spots

Dried Blood Spots

The detection and quantitation of CMV by real-time PCR amplification. PCR detects amplicons of the immediate-early (IE) viral protein 1 (IE1, UL123) and Glycoprotein B (gB, UL55) coding regions. Quantitation of CMV DNA (IU/mL) is achieved by amplifying a standard curve at the same efficiency, consisting of serial dilutions of a known amount of DNA containing the CMV amplicons. Results are reported in International Units per milliliter (IU/mL). The minimum virus level that gives a positive result in 95% of replicates is equivalent to 300 IU/mL (2.48 log IU/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF or Clear non-cellular fluid (e.g. BAL or Washing), see CMV Quantitative by PCR [CMVQN].

For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (e.g. BAL or Washing), see CMV Qualitative by PCR, Tissue/Cells [CMVQLT].

For Eye Fluid, see CMV Quant PCR for Eye Fluids [CMVEYE] or Eye Fluid Viral PCR Quant Panel [EYEVQP].




Real-time Polymerase Chain Reaction.

Reference Range
See individual components

Ordering & Collection

Specimen Type
Dried Blood Spot

Dried Blood Spot: Testing only performed on the original Newborn Screening Card from patient's time of birth. See Specimen Handling to request card from WA DOH.

Fill one circle of a Newborn Screening Card completely with blood (confirm blood has soaked through the card). Label or tag each specimen in English with at least two of the following: patient name, date of birth and/or unique I.D. number corresponding to the request form.

Minimum sample requirement: 1 full circle

Handling Instructions


The original Newborn Screening Card must be obtained from the WA DOH using the In-house Consent Form.

In-house providers must place future orders for testing in EPIC


Obtain the original Newborn Screening Card from the DOH using the CSS/Ref Consent Form.

Once specimen is received, forward to UWMC SPS with Virology Lab Request Form.

requested: 1 full circle
minimum: 1 full circle



Send blood spot to Molecular Virology lab at room temperature.

Transport Temperature


LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Molecular

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102

Tuesday. Results following Thursday.
Available STAT?

Billing & Coding

CPT codes