BK Virus by PCR Quantitative

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General Information

Lab Name
BK Virus PCR Quant
Lab Code
Epic Ordering
BK Virus PCR Quant

Blood (EDTA Plasma)

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the FDA approved Roche cobas® BKV Quantitative assay for the detection of BK polyomavirus (BKV) using real-time PCR amplification.

The lower limit of detection, LoD (the minimum virus level that gives a positive result in 95% of replicates), and the lower limit of quantitation, LLoQ, are both 21.5 IU/mL.

A conversion factor of 1.1 Log10 IU/mL is equivalent to 1.0 Log10 copies/mL.

The Roche cobas® BKV assay uses primers specific for the BKV VP2 region and the BKV small t-antigen region.

For Tissue Biopsy, Bone Marrow, or Cellular fluid (e.g. BAL or Washing), see BK Virus by PCR Qualitative [BKVQL].

For Urine, see BK Virus by PCR Quantitative, Urine [UBKQN].

Polyomavirus Type BK-Quant



Real-time Polymerase Chain Reaction

Reference Range
See individual components
Ref. Range Notes

Not Applicable

Ordering & Collection

Specimen Type

Blood: 3 mL EDTA plasma or PPT

Unacceptable samples: Plasma collected in sodium or lithium heparin.

Note: Separate specimens MUST be submitted when both Serology and PCR testing are ordered.

Handling Instructions

Freshly drawn whole blood specimens may be held at 2 to 25°C for up to 24 hours prior to plasma preparation through centrifugation.

requested: 1.0 mL of EDTA plasma
minimum: 350µL of EDTA plasma



Blood: Centrifuge upon arrival, remove plasma from cells.

Plasma specimens may be stored and/or transported:

  • 2 to 30°C for up to 24 hours
  • 2 to 8°C for up to 6 days
  • -15°C to -80°C for long-term storage up to 6 months.

Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice is unavailable, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 6 days of collection. Do not freeze/thaw.

Transport Temperature


LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Renton

1601 Lind Ave SW
Renton, WA 98057

206-685-6656 opt 4

Tuesday and Friday. Results same day.
Available STAT?

Billing & Coding

CPT codes