CMV Quant PCR for Eye Fluids

The detection and quantitation of CMV by real-time PCR. The PCR detects amplicons of the immediate-early (IE) viral protein 1 (IE1, UL123) and Glycoprotein B (gB, UL55) coding regions. Quantitation of CMV DNA (IU/mL) is achieved by amplifying a standard curve at the same efficiency, consisting of serial dilutions of a known amount of DNA containing the CMV amplicons. Results are reported in International Units per milliliter (IU/mL). The minimum virus level that gives a positive result in 95% of replicates is 300 IU/mL (2.48 log IU/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF or Clear non-cellular fluid (e.g. BAL or Washing) and Swab see CMV Quantitative by PCR [CMVQN].

For Tissue Biopsy, Bone Marrow, or Cellular fluid (e.g. Washing), see CMV Qualitative by PCR, Tissue/Cells [CMVQLT].

For Dried Blood Spot, see CMV Quant by PCR, Blood Spots [CMVBSQ].